Cytoskeletal organization in stage 2 oocytes...
tubulin sidebarGIF Microtubules: During stage II, a dense, apparently disordered, network of MTs fills the oocyte cytoplasm. Low magnification images (JPEG: 25 KB) reveal that, by stage III, MTs  radiate from the perinuclear cytoplasm towards the oocyte cortex. The yolk-free perinuclear cytoplasm also contains an extensive, but apparently disordered array of MTs (JPEG: 38 KB). The centrosomal protein g-TB appears in small foci that are symmetrically distributed between the animal and vegetal cortex (JPEG: 41 KB; JPEG: 31 KB; JPEG: 41 KB).
S2actnlm F-actin: The GV of stage II-III oocytes is brightly stained by fluorescent phalloidin (JPEG: 62 KB), though significant attenuation of the signal is apparent due to the accumulation of yolk. Cortical, subcortical, and cytoplasmic actin cables are also apparent during these stages of oogenesis (not shown).
CK sidebar Keratins: The KF network is further elaborated throughout stages II-III of oogenesis. By late stage I - stage II, oocytes contain a complex, but poorly-ordered network of KFs extending throughout the oocyte cytoplasm (JPEG: 54 KB; QT or AVI: KB). During late stages II - III, the KFs adopt a more radial organization, extending from a poorly organized perinuclear network towards the KF network in the subcortical cytoplasm (JPEG: 115 KB; JPEG: 83 KB; AVI or QT: KB).