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RESEARCH INTERESTS
SV40 transformation
growth control
cancer
genomic instability
DNA rearrangement
monoclonal antibodies against tumor antigens
PUBLICATIONS
I am not currently involvoed in research. The monoclonal
antibodies specific for p53 and for SV40 antigen that
my colleagues and I isolated are available from
PharMingen (www.pharmingen.com)
and are still in use in many laboratories. The research
project that I shared with Theodore Gurney is dormant
now, though it still interests both of us. Our project is
an investigation of spontaneous DNA rearrangements
that occur as short tandem duplications or deletions
within or near the integrated SV40 DNA in mouse
chromosomes. The rate of rearrangement is very high,
greater than 10-3 events/cell/division, 1000 to 10,000
times higher than the usual rate of spontaneous
mutation. The high rate requires the presence of the
SV40 early control region, a 350 nucleotide segment of
SV40 DNA containing the SV40 origin of DNA replication,
one or two enhancers,and a promoter of SV40 T antigen
transcription. In one system, rearrangements required
the SV40 control region but not the T antigen gene or its
expression. In another system the rate of rearrangement
was tightly correlated with the type of T antigen
expressed. Figure 1 shows that the line X1 which
expressed wild-type T antigen rearranged at a higher
rate than did the closely related SVT2 which expressed
a mutant larger T antigen. We propose that the control
region is required for rearrangement but T antigen
expression can modulate the rate of rearrangement.
Selected Publications
Gurney, E.G. and T. Gurney, Jr. (1979) Density-dependent inhibition of both growth and T-antigen expression in revertants isolated from simian virus 40-transformed mouse SVT2 cells. J. Virol. 32:661-671.
Gurney, E.G., R.O. Harrison and J. Fenno (1980) Monoclonal antibodies against simian virus 40 T antigens: Evidence for two distinct sub-classes of large T antigen and for similarities among nonviral T antigens. J. Virol. 34:752-763.
Gurney, E.G., S. Tamowski and W. Deppert (1986) Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T anti-gen. J. Virol. 57:1168-1172.
Gurney, T., Jr., and E.G. Gurney (1989) Spontaneous rearrangement of integrated simian virus 40 DNA in nine transformed rodent cell lines. J. Virol. 63:165-174.
Hunter, D.J. and E.G. Gurney. 1994. The genomic
instability associated with integrated simian virus 40
DNA is dependent on the origin of replication and the
early control region. J. Virol. 68:787-796.
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